Publications
Near-infrared fluorogenic RNA for in vivo imaging and sensing
Z. Chen, W. Chen, C. Xu, H. Song, X. Ji, H. Jiang, H. Duan, Z. Li, W. Gao, T. Yao, Z. Zhang, L. He, Y. Yin, N. Yang, W. Tian, J. Wu, X. Li, Nature Communications, 16, 518 (2025).
T. G. Pham, O. Ajayi, J. Wu, bioRxiv, (2024).
Live-cell imaging of mRNA using a Pepper RNA tag
J. Wu, S. R. Jaffrey, Methods in Molecular Biology, 2875, 1–7 (2024).
Imaging S-Adenosyl methionine dynamics in living cells using an RNA-based fluorescent sensor
J. D. Moon, K. Yusko, L. Nassimos, J. Wu, Methods in Molecular Biology, 2774, 259–267 (2024).
Fluorogenic CRISPR for genomic DNA imaging
Z. Zhang, X. Rong, T. Xie, Z. Li, H. Song, S. Zhen, H. Wang, J. Wu, S. R. Jaffrey, X. Li, Nature Communications, 15, 934 (2024).
Recent advances in methods for live-cell RNA imaging
T. G. Pham, J. Wu, Nanoscale, 16, 5537–5545 (2024).
Genetically encoded RNA-based sensors with Pepper fluorogenic aptamer
Z. Chen, W. Chen, Z. Reheman, H. Jiang, J. Wu, X. Li, Nucleic Acids Research, 51, 8322–8336 (2023).
Prior publications
L. F. M. Passalacqua, M. R. Starich, K. A. Link, J. Wu, J. R. Knutson, N. Tjandra, S. R. Jaffrey, A. R. Ferré-D’Amaré, Nature Communications, 14, 2969 (2023).
Caspase-11 interaction with NLRP3 potentiates the noncanonical activation of the NLRP3 inflammasome
J. Moretti, B. Jia, Z. Hutchins, S. Roy, H. Yip, J. Wu, M. Shan, S. R. Jaffrey, J. Coers, J. M. Blander, Nature Immunology, 23, 705–717 (2022).
Detection of SARS-CoV-2 RNA using a DNA aptamer mimic of green fluorescent protein
B. S. VarnBuhler, J. Moon, S. K. Dey, J. Wu, S. R. Jaffrey, ACS Chemical Biology, 17, 840–853 (2022).
Self-assembly of intracellular multivalent RNA complexes using dimeric Corn and Beetroot aptamers
J. Wu, N. Svensen, W. Song, H. Kim, S. Zhang, X. Li, S. R. Jaffrey, Journal of the American Chemical Society, 144, 5471–5477 (2022).
Naturally occurring three-way junctions can be repurposed as genetically encoded RNA-based sensors
J. D. Moon, J. Wu, S. K. Dey, J. L. Litke, X. Li, H. Kim, S. R. Jaffrey, Cell Chemical Biology, 28, 1569–1580.e4 (2021).
Engineering fluorophore recycling in a fluorogenic RNA aptamer
X. Li, J. Wu, S. R. Jaffrey, Angewandte Chemie, 133, 24355–24363 (2021).
L. Tang, S. Zhang, Y. Zhao, N. D. Rozanov, L. Zhu, J. Wu, R. E. Campbell, C. Fang, International Journal of Molecular Sciences, 22, 445 (2021).
Far-red fluorescent genetically encoded calcium ion indicators
R. Dalangin, M. Drobizhev, R.S. Molina, A. Aggarwal, R. Patel, A. S. Abdelfattah, Y. Zhao, J. Wu, K. Podgorski, E.R. Schreiter, T. E. Hughes, R. E. Campbell, Y. Shen, bioRxiv, (2020).
Imaging mRNA trafficking in living cells using fluorogenic proteins
J. Wu, S. R. Jaffrey, Current Opinion in Chemical Biology, 57, 177–183 (2020).
X. Li, H. Kim, J. L. Litke, J. Wu, S. R. Jaffrey, Angewandte Chemie, 59, 4511–4518 (2020).
Live imaging of mRNA using RNA-stabilized fluorogenic proteins
J. Wu, S. Zaccara, D. Khuperkar, H. Kim, M. E. Tanenbaum, S. R. Jaffrey, Nature Methods, 16, 862–865 (2019).
Understanding the fluorescence change in red genetically encoded calcium ion indicators
R. S. Molina, Y. Qian, J. Wu, Y. Shen, R. E. Campbell, M. Drobizhev, T. E. Hughes, Biophysical Journal, 116, 1873–1886 (2019).
A bioluminescent Ca2+ indicator based on a topological variant of GCaMP6s
Y. Qian, V. Rancic, J. Wu, K. Ballanyi, R. E. Campbell, ChemBioChem, 20, 516–520 (2019).
A genetically encoded near-infrared fluorescent calcium ion indicator
Y. Qian, K. D. Piatkevich, B. Mc Larney, A. S. Abdelfattah, S. Mehta, M. H. Murdock, S. Gottschalk, R. S. Molina, W. Zhang, Y. Chen, J. Wu, M. Drobizhev, T. E. Hughes, J. Zhang, E. R. Schreiter, S. Shoham, D. Razansky, E. S. Boyden, R. E. Campbell, Nature Methods, 16, 171–174 (2019).
Genetically encoded glutamate indicators with altered color and topology
J. Wu, A. S. Abdelfattah, H. Zhou, A. Ruangkittisakul, Y. Qian, K. Ballanyi, R. E. Campbell, ACS Chem Biology, 13, 1832–1837 (2018).
Y. Shen, Y. Chen, J. Wu, N. C. Shaner, R. E. Campbell, PLoS One, 12, e0171257 (2017).
Tracking translation one mRNA at a time
J. Wu, S. R. Jaffrey, Nature Biotechnology, 34, 723–724 (2016).
Engineering dark chromoprotein reporters for photoacoustic microscopy and FRET imaging
Y. Li, A. Forbrich, J. Wu, P. Shao, R. E. Campbell, R. Zemp, Scientific Reports, 6, 22129 (2016).
J. Wu, D. L. Prole, Y. Shen, Z. Lin, A. Gnanasekaran, Y. Liu, L. Chen, H. Zhou, S. R. W. Chen, Y. M. Usachev, C. W. Taylor, R. E. Campbell, Biochemical Journal, 464, 13–22 (2014).
A long Stokes shift red fluorescent Ca2+ indicator protein for two-photon and ratiometric imaging
J. Wu, A. S. Abdelfattah, L. S. Miraucourt, E. Kutsarova, A. Ruangkittisakul, H. Zhou, K. Ballanyi, G. Wicks, M. Drobizhev, A. Rebane, E. S. Ruthazer, R. E. Campbell, Nature Communications, 5, 5262 (2014).
Palmitoylation is the switch that assigns calnexin to quality control or ER Ca2+ signaling
E. M. Lynes, A. Raturi, M. Shenkman, C. Ortiz Sandoval, M. C. Yap, J. Wu, A. Janowicz, N. Myhill, M. D. Benson, R. E. Campbell, L. G. Berthiaume, G. Z. Lederkremer, T. Simmen, Journal of Cell Science, 126, 3893–3903 (2013).
J. Wu, L. Liu, T. Matsuda, Y. Zhao, A. Rebane, M. Drobizhev, Y-F. Chang, S. Araki, Y. Arai, K. March, T. E. Hughes, K. Sagou, T. Miyata, T. Nagai, W-H. Li, R. E. Campbell, ACS Chemical Neuroscience, 4, 963-972 (2013).
S. C. Alford, J. Wu, Y. Zhao, R. E. Campbell, T. Knöpfel, Biology of the Cell, 105, 14–29 (2013).
An expanded palette of genetically encoded Ca2+ indicators
Y. Zhao, S. Araki, J. Wu, T. Teramoto, Y-F. Chang, M. Nakano, A. S. Abdelfattah, M. Fujiwara, T. Ishihara, T. Nagai, R. E. Campbell, Science, 333, 1888–1891 (2011).